Forum :: effect of ligand surface density on the apparent k


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: effect of ligand surface density on the apparent k

effect of ligand surface density on the apparent k 14 Mar 2006 01:00 #1

Hi,
What is that somebody could help me?
What is the effect of the rate of immobilization of the ligand on the ka, kd and KD?
thanks

effect of ligand surface density on the apparent k 14 Mar 2006 01:00 #2

What is the effect of the rate of immobilization of the ligand on the ka, kd and KD?

I think you mean:

What is the effect of the immobilization density of the ligand on the ka, kd and KD?

In general a high immobilization density gives rise to all kinds of side effects masking the true kinetics of your system.

In steady state the KD is the result of all these interactions.

The association phase will probably not be 1:1 and the high ligand density favours rebinding during dissociation.

For kinetics the lowest ligand density with a good response (say 100 RU) is the best.

Arnoud