Direct immobilization - Forum


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.
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TOPIC: Direct immobilization

Direct immobilization 02 Jan 2013 01:00 #1

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Hello.

I have always used capture immobilization. It is the first time I'm going to do a Direct immobilization on a CM5 chip. Will use Amine coupling. My Ligand mol. wt. is ~28000. I want to have an RL of approx. 200 RU. Any suggestions on; 1) concentration of ligand to start? 30 ug/mL:? 2) Suggested contact time for the ligand; 3) Suggested time for NHS/EDC activation? 4) How long to block with Ethanolamine. Kindly comment. Thanks.
mtarca

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Direct immobilization 02 Jan 2013 01:00 #2

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Hi,

Did you read the pages www.sprpages.nl/immobilization.html ?

1) start with a pre-concentration experiment and 10 - 30 g/ml
2) depends on the immobilization speed
3) start with 7 minutes
4) 7 minutes

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Last Edit: by MQAdmin03.
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