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- Can I fitting curve using langmuir isotherm without Keq?
- Units and conversions
- Plasmon-based sensing outside the laboratory
- Is this similar to kinetic titration, right?
- Unspecific Binding in Reference Cell of Biotin CAPture chip
- Looking for Used Biacore 2000 or 3000
- Need help- Biacore data analysis
- Biacore Rmax vs RelResp
- I soluted 'unknown band disappera' phenomena.
- Negative curves
- Some trouble can prevent just blocking bubble?
- antibody-virus interaction Lazy antibody...
- What's the situation, disappearing during the SPR experiment?
- What happened in this plot...?
- How can we make these different channels in one?
- Line plot is NOT beautiful...
- Sigmoidal binding curver?
- Bubbles in the system
- export SPR curves
- Unusual sensorgram : dissociation below baseline
- normalization of SPR DATA
- Au NPs coating of a surface for interaction with green light
- DNA and carry over problems
- sensorgram and curve
- supplier of 50 nm gold film on BK7 substrates
- Single Cycle Kinetics
- Proper Fitting Model
- Biacore T100
- Pre-concentration
- Relating to SPR buffer..DMSO
- CM dextran chip protocol
- Bare Gold chip Amine coupling
- big drop at the start of dissociation
- RU response to concentration
- Binding curves for monoclonal antibodies
- Competitive SPR with serum antibodies
- How can I solute reference channel's curve?
- NTA chip and amine coupling
- Which method reduces negative curve by buffer conc. mismatch?
- increase in ka
- negative curve by mismatch
- NTA Chip Regeneration Issues
- Long spikes before association and after dissociation
- Streptavidin adsorption and binding to carboxylated chip
- Baseline increases between cycles
- please help
- fresh SPR user need help for weird signal after injection
- Error during startup
- What Binding Kinetics Can Tell You That Affinity Can't.
- Stability values in negatives
- New LinkedIn Group- Surface Plasmon Resonance
- Do you have your own personal SPR?
- How Can You save on Precious Protein Samples?
- HIS capture leaching
- LSPR vs. SPR: What's the Difference?
- Dissociation buffer
- CM5 Lifetime
- Sensor Chip Au
- Long spike after injection
- Capture level decrease
- Biosensor coupling, chemistry problems...
- How Much Protein Do You Really Have?
- How Preconcentration Can Save Your Protein Samples in SPR Exper
- Inconsistancy in results
- Increasing sensorgram in dissociation phase
- Negative RU upon analyte injection
- uniform dissociation
- Problems with immobilisation
- Spikes with injection
- Streptavidin capturing and small molecules
- SPR validation
- Determination of Ka and Kd
- Streptavidin Chip
- Multivalent Binding
- bScreen LB 991 Label-free & Microarray Reader
- Homemade set-up
- small oscillation in the beginning of the sensorgram
- Reducing non-specific binding in SPR
- Scrubber2 reference question
- Dissociation below baseline
- Sensorgram increase at the start
- variation in kinetic parameters
- How to image reflected light of wavelength 635 nm 5 mW?
- SPR can Uncover What Lies Beneath the Surface
- Job Opportunity- Nicoya is looking for independent sales agents
- Curve Fitting
- Conditioning NTA chip and Ni not loading on chip
- Protease Inhibitor
- Replicates and RSD% on fitted parameters
- Genetically engineered antibody: antibody drugs come into th
- Random Spikes during run/ immobilization
- Kinetic model
- Immobilization issues!
- Less point in dissiciation phase
- SPR
- How to Become an SPR Superhero - SENSORCON ONE Top 5
- BIAcore X100 instrument opinions
- Sensorgrams - inaccurate fits
- Biacore S200
- No signal during immobilization scouting
- Binding values I get with zero buffer
- Double peak
- Dipping curves
- Anti His methodology reference flow cell prep
- Storage and Re-use of BIotin CAPture Chips
- pH scouting - No complete dissociation.
- Chi2- something interesting i read!
- Low response during secondary binding using NHS/EDC
- does anyone have a problem with negative curve...?
- when should we set RI=0 for a 1:1 fit?
- Rmax is variable ?
- Determination of KD from inhibition assay steady state data
- Biacore replacement
- Determination of protein concentration on the chip
- Temperature Problem
- Reconditioning of CM5 chips
- Reference channel Question
- Shape of the 'dips' in SPR
- Measuring avidity
- Problems with capturing on NTA chip
- SPR on Kinases
- SensiQ users
- immobilization of DNA onto a C1 chip
- Replacement parts-syringe- where to get
- Reconditioning CM5 chips
- Comparison between SPR and AUC techniques
- Chi2
- capture immobilization levels on a antiHis antibody
- Low protein signal during immobilisation
- Looking for a SPR contract lab near Bay area
- regeneration conditions
- Odd kinetic trace
- Norton says Scrubber.exe not safe
- Clamp XP
- GST tag-nonspecific biding?
- The shape of sensorgrams
- Nanoparticles in a SPR device?
- Negative binding activity
- SPR and LSPR
- Shear stress during SPR
- getting fine-grain data
- binding behavior as a function of polarity
- reproducibility of sensograms and general data quality
- Protein immobilization
- Immobilization with mPEG-NH2 in stead of EtOH-NH2
- buffer with EDTA
- immobilization/ amine coupling
- Declining binding signal during injection
- How to add an image to your post
- Read this first
- Old forum
- Nanoparticles
- DMSO a small molecules
- Read this first
- Protein-small inhibitors interaction
- Sensorgram doubt
- Heterogeneous ligand model - which parameter is the correct one
- Streptavidin on to CM5 chip
- Hepes concentration
- ka calculation problem
- Immobilization?
- Literature about bivalent interaction and concentration
- Buffer exchange for SPR
- Rmax question
- analyte recovery/ligand fishing
- Problems with immobilisation
- capture approach / pre steady state approach
- 1:1 / 2:1 stoichiometry
- Streptavidin nonspecific in matrix
- Bulk change due to the change of RI
- Negative curves but not inverted
- Adding Neutravidin to CM5 chip
- Direct immobilization
- Protein G
- Antibody measurements in serum
- Instrument error
- Biphasic curve
- Curve fitting
- Inverted curve
- Signal decreasing during association phase
- Vials for reichert 7000
- Dissociation phase and injection time issues
- Linkage chemistries
- Xantec chip
- Signal decreasing during injection
- Phage screening and regeneration
- Non-specific binding/data modeling issue
- Reference surface
- Problems with ClampXP cant set injection propertie
- Streptavidin immobilisation
- Immobilisation level
- Plant protein interaction
- Bulk contribution messages
- Control variation
- NSB reducer
- Regen solution for strepavidin
- Regeneration acceptability
- Tannin binding
- Inverted curve - continued
- concentration dependent negative response
- Amine coupling in TRIS
- small molcule binding
- Poor fit
- Ethanolamine
- Non-specific binding
- chip variation and life
- RU response into mass density
- Negative response units????
- non specific binding issues
- Regeneration issues
- Immobilization level
- RU increase upon injection finish
- Peptides
- CM3 virus
- SPR dissociation curve deviation
- Chip preconditioning
- RU decreasing during injection
- DNA-SH immobilisation
- Homemade SPR system
- Strange high responses
- No detection/bulk on FC1 but Fc2 works perfectly
- Use different chip as a reference cell
- Protocols
- Rapid decrease after injection
- problems coupling anti-GST
- L1 chips
- Rmax global or local?
- Whole cells and aptamers
- Kinetic simulation
- Air pressure and BiacoreX
- Biacore X and requirements for setup
- How to get the upper asymtote/limit of detection
- SPR interaction for small molecules
- Small molecule-RNA aptamer SPR
- Kinetic software
- Analyte binding to chip surface
- Biotin-tagged molecule immobilisation
- Ligand amount
- Thiol coupling
- Glycerol in main buffer
- small molecule immobilisation
- More on negative sensorgrams
- Amine coupling
- General question about kinetic
- Regeneration of Biosensor chip
- Complex kinetics
- New, novel method for gold-plating SPR chips
- SPR residuals
- K on K off values
- Other buffer problems
- buffer problems
- Help! SPR in self-complementary DNA
- Can Sulfo-NHS displace NHS?
- why thiol coupling won't work for oligonucleoti
- Antibody-Antigen
- Help - biotinylated peptide does not bind to CM
- advantages of SPR over FRET
- Orientation strategy question
- why signal always jumped on one fc
- Removing ligand
- Thiol - antibody immobilization
- Injection blocked
- ligand immobilization
- effect of ligand surface density on the apparent k
- Unspecific signals
- How to "zero"data in BiaEval easily?
- Linked reactions
- calibration drift/instability
- Interaction only at slow flow rate ?
- Opto-interface
- Thickness and angular shift in SPR
- Langmuir 1:1 kinetics
- negative binding signals
- Global or single fitting ?
- Inverted curve
- General discussion
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