Please read the tutorial before making the quiz. All the answers are 'hidden' in the text.
At the end of the quiz, you can give your name and email address to receive the results. The quiz has 30 questions and will take 5 - 10 minutes to complete. Read the questions carefully.
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Q 1:
Which sensorgram has exponential interaction curves?
A B C D
Q 2:
Which curve is an exponential interaction curve with mass-transfer?
A B C D
Q 3:
Which sensorgram does not have exponential interaction curves?
A B C D
Q 4:
What is wrong with this sensorgram?
A: the axis legends are missing
B: there are no replicates
C: the bulk effect is too big
D: not all curves go to steady state
Q 5:
What is wrong with this sensorgram?
A: the axis legends are missing
B: there are no replicates
C: the concentration range is too narrow
D: not all curves go to steady state
Q 6:
What is wrong with this sensorgram?
A: the axis legends are missing
B: there are no replicates
C: the concentration range is too narrow
D: not all curves go to steady state
Q 7:
What should be solved first before fitting?
A: association time should be longer
B: these are not exponentials: perform a better experiment
C: dissociation time should be longer
D: match flow and analyte buffer
Q 8:
Which sensorgram can you use for equilibrium analysis?
A B C D
Q 9:
What shoud be optimized before fitting?
A: association time longer
B: longer dissociation time
C: user higher analyte concentrations
D: use lower analyte concentrations
Q 10:
Which curve has the fastest dissociation rate constant?
A: curve A
B: curve B
C: you should know the analyte concentration
D: you should know the Rmax of the system
Q 11:
Which curve has the fastest association rate constant?
Hint: What determines the association rate.
A: curve A
B: curve B
C: you should know the analyte concentration
D: you should know the Rmax of the system
Q 12:
Which curve has the fastest association rate constant?
A: curve A
B: they are all the same
C: curve D
D: C is higher than D
Q 13:
Which curve has the highest equilibrium constant?
A: curve A
B: they are all the same
C: curve D
D: C is higher than D
Q 14:
Which fitting result should you report?
A B C D
Q 15:
Which parameters should be globally fitted?
A: ka, kd, KD, Rmax
B: ka, kd, Rmax
C: ka, kd, Rmax, RI
D: Rmax, RI, Chi2
Q 16:
What can you do when you have this sensorgram?
A: check analyte for purity
B: reverse the ligand and analyte
C: A + B
D: make the flow faster
Q 17:
What can you do when you have this sensorgram?
A: check the analyte for purity
B: reverse the analyte and ligand
C: try another immobilisation technique
D: A + B + C
Q 18:
What can you do to optimize this interaction?
| 1: | lower ligand concentration |
| 2: | match buffers better |
| 3: | equilibrate better |
| 4: | use higher flow rate |
A: 1 + 2
B: 3 + 4
C: 1 + 2 + 3
D: 2 + 3 + 4
Q 19:
How much ligand should you immobilize for analyte concentration measurements?
A B C D
Q 20:
How much ligand should you immobilize for kinetic analysis?
A B C D
Q 21:
Which curve is in equilibrium (steady state)?
A: curve A
B: curve B
C: curve C
D: all four curves
Q 22:
Which curve is saturating the ligand?
A: curve A
B: curve B
C: curve C
D: all four curves
Q 23:
Which curve has an analyte concentration comparable to the KD?
A: curve A
B: curve B
C: curve C
D: all four curves
Q 24:
When you have this fit as a result. What can you do?
Hint: go for the best result.
| 1: | lower ligand concentration |
| 2: | match buffers better |
| 3: | check the ligand for purity |
| 4: | use higher flow rate |
A: 1 + 2
B: 1 + 3
C: 2 + 4
D: 1 + 3 + 4
Q 25:
Are the values given in the inset plausible with this sensorgram?
Hint: look at the curve spacing.
A: yes
B: no, dissociation looks faster
C: no, dissociations looks slower
D: no, Rmax is too high
Q 26:
What do you want to change if you see this fitting?
A: nothing, this looks fine
B: make association time longer
C: make dissociation time longer
D: use higher analyte concentration
Q 27:
To calculate (fit) meaningful results you need curves
A: which go to Rmax
B: which go to steady state
C: which have curvature
D: with a low response
Q 28:
What can you tell about the Rmax?
A: it is dependent on the ka and kd of an interaction
B: it is dependent on the surface capacity and the molecular weight of ligand and analyte
C: it is dependent on the analyte concentration
D: B + C
Q 29:
What can you say about this sensorgram?
A: the analyte concentration range is not wide enough
B: the response is not following exponential kinetics
C: there is mass transport limitation
D: this looks a fine sensorgram
Q 30:
The minimal requirements in a publication are:
A: sensorgram + fit overlay + kinetic values
B: sensorgrams (replicates) + fit overlay + kinetic values
C: table with kinetic values and representative sensorgram
D: full method used in the experiments + B
