Please read the tutorial before making the quiz. All the answers are 'hidden' in the text.
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Q 1:

Which sensorgram has exponential interaction curves?
A B C D
Q 2:

Which curve is an exponential interaction curve with mass-transfer?
A B C D
Q 3:
Which sensorgram does not have exponential interaction curves?
A B C D
Q 4:
What is wrong with this sensorgram?
A: the axis legends are missing
B: there are no replicates
C: the bulk effect is too big
D: not all curves go to steady state
Q 5:
What is wrong with this sensorgram?
A: the axis legends are missing
B: there are no replicates
C: the concentration range is too narrow
D: not all curves go to steady state
Q 6:
What is wrong with this sensorgram?
A: the axis legends are missing
B: there are no replicates
C: the concentration range is too narrow
D: not all curves go to steady state
Q 7:
What should be solved first before fitting?
A: association time should be longer
B: these are not exponentials: perform a better experiment
C: dissociation time should be longer
D: match flow and analyte buffer
Q 8:
Which sensorgram can you use for equilibrium analysis?
A B C D
Q 9:
What shoud be optimized before fitting?
A: association time longer
B: longer dissociation time
C: user higher analyte concentrations
D: use lower analyte concentrations
Q 10:
Which curve has the fastest dissociation rate constant?
A: curve A
B: curve B
C: you should know the analyte concentration
D: you should know the Rmax of the system
Q 11:

Which curve has the fastest association rate constant?
Hint: What determines the association rate.
A: curve A
B: curve B
C: you should know the analyte concentration
D: you should know the Rmax of the system
Q 12:
Which curve has the fastest association rate constant?
A: curve A
B: they are all the same
C: curve D
D: C is higher than D
Q 13:
Which curve has the highest equilibrium constant?
A: curve A
B: they are all the same
C: curve D
D: C is higher than D
Q 14:
Which fitting result should you report?
A B C D
Q 15:
Which parameters should be globally fitted?
A: ka, kd, KD, Rmax
B: ka, kd, Rmax
C: ka, kd, Rmax, RI
D: Rmax, RI, Chi2
Q 16:
What can you do when you have this sensorgram?
A: check analyte for purity
B: reverse the ligand and analyte
C: A + B
D: make the flow faster
Q 17:
What can you do when you have this sensorgram?
A: check the analyte for purity
B: reverse the analyte and ligand
C: try another immobilisation technique
D: A + B + C
Q 18:
What can you do to optimize this interaction?
1: | lower ligand concentration |
2: | match buffers better |
3: | equilibrate better |
4: | use higher flow rate |
A: 1 + 2
B: 3 + 4
C: 1 + 2 + 3
D: 2 + 3 + 4
Q 19:
How much ligand should you immobilize for analyte concentration measurements?
A B C D
Q 20:
How much ligand should you immobilize for kinetic analysis?
A B C D
Q 21:
Which curve is in equilibrium (steady state)?
A: curve A
B: curve B
C: curve C
D: all four curves
Q 22:
Which curve is saturating the ligand?
A: curve A
B: curve B
C: curve C
D: all four curves
Q 23:

Which curve has an analyte concentration comparable to the KD?
A: curve A
B: curve B
C: curve C
D: all four curves
Q 24:

When you have this fit as a result. What can you do?
Hint: go for the best result.
1: | lower ligand concentration |
2: | match buffers better |
3: | check the ligand for purity |
4: | use higher flow rate |
A: 1 + 2
B: 1 + 3
C: 2 + 4
D: 1 + 3 + 4
Q 25:

Are the values given in the inset plausible with this sensorgram?
Hint: look at the curve spacing.
A: yes
B: no, dissociation looks faster
C: no, dissociations looks slower
D: no, Rmax is too high
Q 26:

What do you want to change if you see this fitting?
A: nothing, this looks fine
B: make association time longer
C: make dissociation time longer
D: use higher analyte concentration
Q 27:
To calculate (fit) meaningful results you need curves
A: which go to Rmax
B: which go to steady state
C: which have curvature
D: with a low response
Q 28:
What can you tell about the Rmax?
A: it is dependent on the ka and kd of an interaction
B: it is dependent on the surface capacity and the molecular weight of ligand and analyte
C: it is dependent on the analyte concentration
D: B + C
Q 29:

What can you say about this sensorgram?
A: the analyte concentration range is not wide enough
B: the response is not following exponential kinetics
C: there is mass transport limitation
D: this looks a fine sensorgram
Q 30:
The minimal requirements in a publication are:
A: sensorgram + fit overlay + kinetic values
B: sensorgrams (replicates) + fit overlay + kinetic values
C: table with kinetic values and representative sensorgram
D: full method used in the experiments + B