Sensorgrams

Please read the pages above first before making the quiz. All the answers are 'hidden' in the text.

At the end of the quiz, you can give your name and email address to receive the results. The quiz will take 5 - 10 minutes to complete. You can make the quiz as often as you like.

Success!

1. Question 01

Which sensorgram has exponential interaction curves?

A     B     C     D



2. Question 02

Which curve is an exponential interaction curve with mass-transfer?

A     B     C     D



3. Question 03

Which sensorgram does not have exponential interaction curves?

A     B     C     D



4. Question 04

What is wrong with this sensorgram?

A: the axis legends are missing
B: there are no replicates
C: the bulk effect is too big
D: not all curves go to steady state



5. Question 05

What is wrong with this sensorgram?

A: the axis legends are missing
B: there are no replicates
C: the concentration range is too narrow
D: not all curves go to steady state



6. Question 06

What is wrong with this sensorgram?

A: the axis legends are missing
B: there are no replicates
C: the concentration range is too narrow
D: not all curves go to steady state



7. Question 07

What should be solved first before fitting?

A: association time should be longer
B: these are not exponentials: perform a better experiment
C: dissociation time should be longer
D: match flow and analyte buffer



8. Question 08

Which sensorgram can you use for equilibrium analysis?

A     B     C     D



9. Question 09

What shoud be optimized before fitting?

A: association time longer
B: longer dissociation time
C: user higher analyte concentrations
D: use lower analyte concentrations



10. Question 10

Which curve has the fastest dissociation rate constant?

A: curve A
B: curve B
C: you should know the analyte concentration
D: you should know the Rmax of the system



11. Question 11

Which curve has the fastest association rate constant?
Hint: What determines the association rate.

A: curve A
B: curve B
C: you should know the analyte concentration
D: you should know the Rmax of the system



12. Question 12

Which curve has the fastest association rate constant?

A: curve A
B: they are all the same
C: curve D
D: C is higher than D



13. Question 13

Which curve has the highest equilibrium constant?

A: curve A
B: they are all the same
C: curve D
D: C is higher than D



14. Question 14

Which fitting result should you report?

A     B     C     D



15. Question 15

What is bad in this sensorgram presentation?

A: injection time too long
B: concentration range not balanced
C: response too high
D: not all curves reach steady state



16. Question 16

What can you do when you have this sensorgram?

A: check analyte for purity
B: reverse the ligand and analyte
C: A + B
D: inject analyte for a longer time
E: make the flow faster
F: there is nothing wrong with the curves



17. Question 17

What can you do when you have this sensorgram?

A: check the analyte for purity
B: reverse the analyte and ligand
C: try another immobilisation technique
D: A + B + C
E: there is nothing wrong with the curves
this will never work



18. Question 18

What can you do to optimize this interaction?

A: lower ligand concentration
B: match buffers better
C: equilibrate better
D: use higher flow rate
E: A + B + C
F: B + C + D



19. Question 19

How much ligand should you immobilize for analyte concentration measurements?

A     B     C     D



20. Question 20

How much ligand should you immobilize for kinetic analysis?

A     B     C     D



21. Question 21

Which curve is in equilibrium (steady state)?

A: curve A
B: curve B
C: curve C
D: curve D
E: all four curves



22. Question 22

Which curve is saturating the ligand?

A: curve A
B: curve B
C: curve C
D: curve D
E: all four curves



23. Question 23

Which curve has an analyte concentration comparable to the KD?

A: curve A
B: curve B
C: curve C
D: curve D
E: all four curves



24. Question 24

When you have this fit as a result. What can you do?
Hint: go for the best result.

A: lower ligand concentration
B: match buffers better
C: check the ligand for purity
D: use higher flow rate
E: A + C
F: A + D
G: B + D



25. Question 24

Are the values given in the inset plausible with this sensorgram?
Hint: look at the curve spacing.

A: yes
B: no, dissociation looks faster
C: no, dissociations looks slower
D: no, Rmax is too high



26. Question 26

What do you want to change if you see this fitting?

A: nothing, this looks fine
B: make association time longer
C: make dissociation time longer
D: lower ligand concentration
E: use higher analyte concentration



27. To calculate (fit) meaningful results you need curves

A: which go to Rmax
B: which go to steady state
C: which have curvature
D: with a low response



28. What can you tell about the Rmax?

A: it is dependent on the ka and kd of an interaction
B: it is dependent on the surface capacity and the molecular weight of ligand and analyte
C: it is dependent on the analyte concentration
D: it is dependent on the equilibrium constant KD
E: B + C



29. Question 29

What can you say about this sensorgram?

A: the analyte concentration range is not wide enough
B: the response is not following exponential kinetics
C: there is mass transport limitation
D: this looks a fine sensorgram



30. The minimal requirements in a publication are:

A: sensorgram + fit overlay + kinetic values
B: sensorgrams (replicates) + fit overlay + kinetic values
C: table with kinetic values and representative sensorgram
D: full method used in the experiments + B



User Details
Name: (required)
Email:
Country
Send me my Results

How many legs on a typical dog? (e.g: 5)