Sensorgrams

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1.

Which sensorgram has exponential interaction curves?

2.

Which curve is an exponential interaction curve with mass-transfer?

3.

Which sensorgram **does not** have exponential interaction curves?

4.

What is wrong with this sensorgram?

5.

What is wrong with this sensorgram?

6.

What is wrong with this sensorgram?

7.

What should be solved first before fitting?

8.

Which sensorgram can you use for equilibrium analysis?

9.

What shoud be optimized before fitting?

10.

Which curve has the fastest dissociation rate constant?

11.

Which curve has the fastest **association** rate constant?

Hint: What determines the association rate.

12.

Which curve has the fastest association rate constant?

13.

Which curve has the highest equilibrium constant?

14.

Which fitting result should you report?

15.

What is bad in this sensorgram presentation?

16.

What can you do when you have this sensorgram?

17.

What can you do when you have this sensorgram?

18.

What can you do to optimize this interaction?

19.

How much ligand should you immobilize for analyte concentration measurements?

20.

How much ligand should you immobilize for kinetic analysis?

21.

Which curve is in equilibrium (steady state)?

22.

Which curve is saturating the ligand?

23.

Which curve has an analyte concentration comparable to the *K*_{D}?

24.

When you have this fit as a result. What can you do?

Hint: go for the best result.

25.

Are the values given in the inset plausible with this sensorgram?

Hint: look at the curve spacing.

26.

What do you want to change if you see this fitting?

27. To calculate (fit) meaningful results you need curves

28. What can you tell about the Rmax?

*k*_{a} and *k*_{d} of an interaction*K*_{D}

29.

What can you say about this sensorgram?

30. The minimal requirements in a publication are:

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How many legs on a typical dog? (e.g: 5)