SPR-Pages

pagelogo
back
Bio-Rad sensor chips
back

ProteOn sensor chips

Bio-Rad has discontinued the production of the ProteOn XPR36 and its sensor chips. XanTec offers now sensor chips which are compatible with the ProteOn XPR36 system. Both 2D and 3D hydrogel surfaces with different modifications and functionalities are available. The table below shows XanTec's equivalents to the Bio-Rad sensor chips.

ProteOn sensor chips by Xantec
ModificationBio-RadXanTec
short matrix, normal capacityGLCCMD50L, HC30M
Alternative: AL (alginate surface)
normal matrix, low capacityHLC200M
normal matrix, normal capacityGLMCMD200M, CMD500L, HC200M
normal matrix, high capacityCMD700M, HC1000M
long matrix, high capacityGLHHC1500M
no matrix, low capacityCMDP / HCP
poly HIS binding groupHTG / HTEHigh affinity: NiHC1500M, NiHC200M
Standard affinity: NiD200M
hydrophobic surfaceHPP
lipid capturing surfaceGLC + lipid kitLD (hydrogel) & LP (2D)
lipid capturing surface (DNA based)LCP
biotin capturing surfaceNLCStreptavidin: SAD200M & others
Neutravidin: NAHC200M & others
biotin capture surface (DNA based)
biotin capture surface (HIS-tag based)HTG / HTE + HIS-StreptavidinHigh affinity: NiHC1500M, NiHC200M
Standard affinity: NiD200M
plain gold surfaceAU
click chemistryAzide surface: AZHC200M & others
DBCO surface: DCD200M & others
zwitterionic surfaceZC30M, ZC80M, ZC150D
streptavidin capture surfaceBHC30M, BD200M & others
Xantec sensor chips selection guide

ProteOn sensor chips (discontinued)

ProteOn sensor chips are prepared with a modified alginate polymer bound to a gold surface. The alginate layer is attached in different structures to provide surfaces with different binding capacities. The hydrophilic nature of the alginate layer creates a solution-like environment that prevents denaturation of the immobilized ligand and nonspecific adsorption of the analyte. The carboxyl groups on the alginate molecule are designed for easy activation with the ProteOn amine coupling kit using proprietary technology. Because of the increased activation yield on the ProteOn sensor chips the alginate layer is thinner than the dextran layer of other sensor chip types. In fact the GLC sensor chip layer thickness is close to 10 nm.
ProteOn sensor chips provide well-characterized surface chemistries optimized to control ligand immobilization densities and maintain high ligand activities suitable for detailed kinetic analysis as well as qualitative screening applications. Each ProteOn sensor chip is enclosed in a bar-coded cartridge for data tracking and archiving (1).

Sensor chip comparison
modificationBiacoreBio-Rad
short matrix, normal capacityCM3GLC
normal matrix, low capacityCM4
normal matrix, normal capacityCM5GLM
normal matrix, high capacityCM7
long matrix, high capacityGLH
no matrix, low capacityC1
poly HIS binding groupNTAHTG / HTE
hydrophobic surfaceHPA
lipid capturing surfaceL1GLC + lipid kit
lipid capturing surface (DNA based)LCP
biotin capturing surfaceSANLC
biotin capture surface (DNA based)CAPture kit
biotin capture surface (HIS-tag based)HTG / HTE + HIS-Streptavidin
plain gold surfaceAU

References

(1) Bio-Rad Bio-Rad Website. (2012).

Footnotes

  1. ProteOn XPR 36 is not sold any more