The LCP sensor chip surface (1) is functionalized with NeutrAvidin in a planar configuration that is formed on a self-assembled monolayer. The ProteOn LCP reagent kit activates the chip surface by a biotinylated DNA tag so that the chip is able to capture DNA-labeled lipid assemblies through DNA hybridization (compare to the Biacore Biotin CAPture kit). The reagent kit attaches DNA tags to the liposomes in order to anchor them to the chip surface. It is possible to capture two or more layers of lipid assemblies for additional sensitivity. This method of capturing allows for lipid-based interaction analysis, including the analysis of membrane proteins embedded in an intact lipid assembly.
The ProteOn liposome capturing kit includes the LCP sensor chip, the LCP capturing reagent kit required for using the sensor chip, and lipid modification conditioning solution used to clean and stabilize the chip surface prior to capturing liposomes. The reagent kit includes sufficient reagents for the use of two LCP sensor chips (2).
The ProteOn NLC sensor chip contains NeutrAvidin immobilized to a GLC layer for binding of biotin-labeled molecules (proteins, peptides and nucleic acids). It has the capacity to capture ~2,000 RU of IgG or ~500 RU of DNA. The NLC sensor chip is ideal for immobilizing ligands without amine coupling but requires that the ligand be modified with biotin prior to immobilization. It is suitable for various applications, including DNA-protein and protein-protein interaction analysis.
|(1)||Bio-Rad ProteOn LCP sensor chip. (2012). Goto reference|
|(2)||Bio-Rad Bio-Rad Website. (2012). Goto reference|
|(3)||Bio-Rad Bio-Rad Website Sensor chips. (2012).|