The ProteOn HTG sensor chip features novel tris-NTA (3x NTA) complexes for improved capturing of polyhistidine-tagged proteins. This tris-NTA has a significant more stable interaction compared to the mono-NTA surface. The tris-NTA complexes are attached to a polymer matrix layer and are activated by injection of Ni2+ ions for capturing of polyhistidine-tagged proteins/ligands. The HTG sensor chip has a lower tris-NTA surface density and less nonspecific binding, making it most suitable for various applications including protein-protein and protein-peptide interactions.
Bio-Rad recommends using the ProteOn HTG and HTE reagent kit for optimal activation and regeneration of the HTG sensor chip. This reagent kit includes sufficient reagents for >80 activation and regeneration cycles. (1).
|(1)||Bio-Rad Bio-Rad Website. (2012). Goto reference and select USA.|