The ProteOn HTG sensor chip features novel tris-NTA (3x NTA) complexes for improved capturing of polyhistidine-tagged proteins. This tris-NTA has a significant more stable interaction compared to the mono-NTA surface. The tris-NTA complexes are attached to a polymer matrix layer and are activated by injection of Ni²⁺ ions for capturing of polyhistidine-tagged proteins/ligands. The HTG sensor chip has a lower tris-NTA surface density and less nonspecific binding, making it most suitable for various applications including protein-protein and protein-peptide interactions.

Benefits include:

• Improved binding capacity and stability due to the novel tris-NTA formulation
• Reduced decay of ligand over time – ensures reliable results and no cumbersome software corrections needed
• Capture of polyhistidine-tagged proteins directly from crude media, reducing work-up labor and required sample volumes
• Enhanced efficiency and reduced costs due to surface regeneration ability, providing many ligand-analyte interaction studies on the same chip (typically >10 surface regeneration cycles are possible)
• Surface regeneration combined with One-shot Kinetics technique yields exceptionally high throughput

Bio-Rad recommends using the ProteOn HTG and HTE reagent kit for optimal activation and regeneration of the HTG sensor chip. This reagent kit includes sufficient reagents for >80 activation and regeneration cycles. (1).