The ProteOn HTE sensor chip features novel tris-NTA (3 x NTA) complexes for improved capturing of polyhistidine-tagged proteins. This tris-NTA has a significant more stable interaction compared to the mono-NTA surface. The tris-NTA complexes are attached to an alginate matrix layer and are activated by injection of Ni2+ ions for capturing of polyhistidine-tagged proteins/ligands. The HTE sensor chip is most suitable for protein-peptide, protein-DNA, and protein–small molecule interactions.
Bio-Rad recommends using the ProteOn HTG and HTE reagent kit for optimal activation and regeneration of the HTE sensor chip. This reagent kit includes sufficient reagents for >80 activation and regeneration cycles. (1).
|(1)||Bio-Rad Bio-Rad Website. (2012). Goto reference and select USA.|