In this section, the sensor chips, which are used in the SPR instruments, are discussed. The information is extracted from the data sheets provided with the sensor chips, the BIA-Technology Handbook (1), internet information (2) (3) and the other mentioned references.
|short matrix, normal capacity||CM3||GLC||CMD50, HC30|
|normal matrix, low capacity||CM4||HLCx|
|normal matrix, normal capacity||CM5||GLM||CMD200, 500, HC200|
|normal matrix, high capacity||CM7||CMD500D, HC1500M|
|long matrix, high capacity||GLH||CMD700|
|no matrix, low capacity||C1||CMDP|
|poly HIS binding group||NTA||HTG / HTE||NiHC1000M|
|lipid capturing surface||L1||GLC + lipid kit||LP/LD|
|lipid capturing surface (DNA based)||LCP|
|biotin capturing surface||SA||NLC||SADxx, SAHCx|
|biotin capturing surface (DNA based)||CAPture kit|
|biotin capturing surface (His-tag based)||HTG / HTE + His-Streptavidin|
|plain gold surface||AU||AU|
|biotin on surface||BDx|
|click chemistry surface (azide)||AZHC/AZD|
|click chemistry surface (DBCO)||DCD|
|cell capturing surface||PLY|
The sensor chips of the ProteOn system of Bio-rad vary in capacity by varying the length of the used alginate layer. The dextran based sensor chips of Biacore vary the capacity by changing the percentage of carboxylation of the dextran. The capacity of the XanTec sensor chips is controlled by three parameters: i) spatial distance between the brush structured individual hydrogel chains, ii) hydrogel thickness via the chain length and iii) the charge density by the composition of the polycarbonates (3).
The mentioned manufactures all use their own technique to make the sensor chip and therefore it is difficult to compare the capacity of the sensor chip directly.
|(1)||BIACORE AB- BIACORE Technology Handbook. (1998).|
|(2)||Bio-Rad- Bio-Rad website. (2012). Goto reference|
|(3)||XanTec- XanTec biosensors. (2019). Goto reference|