Sensor chips


In this section, the sensor chips, which are used in the SPR instruments, are discussed. The information is extracted from the data sheets provided with the sensor chips, the BIA-Technology Handbook (1), internet information (2) (3) and the other mentioned references.

Table: Overview of available sensor chips
Modification Biacore Bio-Rad XanTec
short matrix, normal capacity CM3 GLC CMD50, HC30
normal matrix, low capacity CM4 HLCx
normal matrix, normal capacity CM5 GLM CMD200, 500, HC200
normal matrix, high capacity CM7 CMD500D, HC1500M
long matrix, high capacity GLH CMD700
no matrix, low capacity C1 CMDP
poly HIS binding group NTA HTG / HTE NiHC1000M
hydrophobic surface HPA HPP
lipid capturing surface L1 GLC + lipid kit LP/LD
lipid capturing surface (DNA based) LCP
biotin capturing surface SA NLC SADxx, SAHCx
biotin capturing surface (DNA based) CAPture kit
biotin capturing surface (His-tag based) HTG / HTE + His-Streptavidin
plain gold surface AU AU
biotin on surface BDx
click chemistry surface (azide) AZHC/AZD
click chemistry surface (DBCO) DCD
zwitterionic surface ZC/ZCC
cell capturing surface PLY
Chip overview

The sensor chips of the ProteOn system of Bio-rad vary in capacity by varying the length of the used alginate layer. The dextran based sensor chips of Biacore vary the capacity by changing the percentage of carboxylation of the dextran. The capacity of the XanTec sensor chips is controlled by three parameters: i) spatial distance between the brush structured individual hydrogel chains, ii) hydrogel thickness via the chain length and iii) the charge density by the composition of the polycarbonates (3).

The mentioned manufactures all use their own technique to make the sensor chip and therefore it is difficult to compare the capacity of the sensor chip directly.


(1) BIACORE AB- BIACORE Technology Handbook. (1998).
(2) Bio-Rad- Bio-Rad website. (2012). Goto reference
(3) XanTec- XanTec biosensors. (2019). Goto reference