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Solvent Correction 05 May 2022 17:58 #1

  • NewUser123
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Hi, I am somewhat new to the SPR world and would like to get more information regarding solvent correction.
I am currently using a published SPR buffer condition which contain 2% DMSO and 10% Glycerol.
My current solvent correction consist of the identical running buffer with a gradient concentration of DMSO (1.6; 1.8; 2.0; 2.2; 2.4; 2.6%), I am wondering if I should do the same with the glycerol, and if so, what is the common practice? Any reference for further reading would be very appreciated!

Ta,
NewUser123

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Solvent Correction 05 May 2022 19:37 #2

Hi,
Both DMSO and Glycerol should be compensated when the concentration gives too high (+ or – 100 RU) bulk responses. The one reference I could find is this one: Frostell-Karlsson, A., et al. (2000). "Biosensor analysis of the interaction between immobilized human serum albumin and drug compounds for prediction of human serum albumin binding levels." J.Med.Chem. 43(10): 1986-1992.

Kind regards
Arnoud
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