NA chip - Forum


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.
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TOPIC: NA chip

NA chip 05 Aug 2020 15:36 #1

Hello. It is my first time to use NA (Neutravidin chip). Does anyone have any suggestion or experience on what regeneration solution to use? I read somewhere that the NA chip is sensitive to acidic pH? Thanks.

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NA chip 06 Aug 2020 19:45 #2

Neutravidin: en.wikipedia.org/wiki/NeutrAvidin

If you want to regenerate the neutravidin-biotin interaction: this is not possible without damaging the sensor chip.

try searching for regenerable avidin

Kind regards
Arnoud

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NA chip 06 Aug 2020 21:55 #3

Oh sorry, I should have been specific in my question. I was going to use the neutravidin chip instead of strepavidin chip. I was going to immobilize my biotinylated ligand to this NA chip, and then pass on the analyte. I was looking for a regeneration solution to knock off the analyte and leave the biotin-ligand attached to the surface for the next cycle. But since the NA chip is not compatible with acidic solutions, I could not use an acidic regent (ie. glycine-HCL 1.5), but wondering what other solutions I can use. Thanks.

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NA chip 07 Aug 2020 08:20 #4

We prefer the cocktail regeneration method ( www.sprpages.nl/kinetics/regeneration ) since this is more gentle on the ligand.
In general (Neutr)Avidin is fairly stable.

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