measuring kinectics from cell supernants? - Forum


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.
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TOPIC: measuring kinectics from cell supernants?

measuring kinectics from cell supernants? 29 Jul 2020 17:12 #1

I have a hybridoma supernatant with antibody and another hybridoma supernatant with an antigen, Can I measure the binding kinectics without purification?

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measuring kinectics from cell supernants? 30 Jul 2020 08:25 #2

No not like this.
You have at least capture one of the components. You can capture the antibody with a proteine A sensor chip and flow your antigen over.
Arnoud

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