Unusual sensorgram shape - Page 2 - Forum


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.
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TOPIC: Unusual sensorgram shape

Unusual sensorgram shape 04 Aug 2020 17:33 #7

The problem with the immobilizing the anti-His antibody and capturing the ligand is that you don't know how well the surface is blocked. Even when you make a reference and block this surface, it seems to me that you introduce a lot of uncertainty in the surface.
I would recommend immobilizing the ligand directly on the surface even when this means that it is randomly orientated.

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