Dear SPR users,
While performing my experiments to find out the affinity of amino acids with my protein, I've found that the amino acids producing more response from the blank cell than the immobilized cell.
Reference cell producing 100 RU, at the same time protein cell giving only 60 RU.
In this scenario, What are all the precautions we should take care to take a valid results out of SPR?. In particular for low mw molecules/fragment screening?