small molcule binding - Page 2 - Forum


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TOPIC: small molcule binding

small molcule binding 24 Aug 2011 02:00 #7

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Hi Arnoud,
Thank you for all your suggestions I´ll try to lower the the coating level, maybe this will help.
Barbara

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small molcule binding 24 Aug 2011 02:00 #8

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I'm not familiar with the T100 Evaluation software but have you tried analyzing the single curves (i.e. not a global fit with all 4 conc) ? I've gotten screwy nonsensical numbers in the past doing a global fit and it has turned out that only one of the concentrations was throwing the whole fit out of line. When I discovered which concentration was askew, I was able to adjust the region selected for fitting and get a more reasonable global fit.

ProfPlum

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