This page is a round-up of the SPR intstruments currently available. The instruments differ in their way of using the technology and design of bringing the interactants in contact which each other. For a direct jump to the manufacturer, click on the logo's in the text. This list is by no means complete.
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Kitchener, ON, January 20, 2016: Nicoya Lifesciences, a leader in label-free molecular analysis, announced today its newest product, OpenSPR-XT - a fully automated molecular analysis instrument based on the highly successful OpenSPR instrument. OpenSPR is a revolutionary surface plasmon resonance instrument that provides powerful label-free analysis while being the most affordable and compact instrument on the market. Instead of using the traditional gold films for the sensor chip, OpenSPR utilizes highly sensitive gold nanoparticles to give detailed information on nanoscale surface interactions. OpenSPR can be used for many SPR applications including binding affinity, kinetics, detection, surface chemistry and more. It is intuitive, easy to use, and compatible with proteins, antibodies, DNA, peptides, polymers, small molecules, and more. OpenSPR’s unique design also allows it to double as a visible cuvette based spectrophotometer.Visit Nicoya Lifesciences
BioNavis Ltd manufactures Multi-Parametric Surface Plasmon Resonance instruments that provide high content information on molecular binding, but also at the same time on nanolayer and nanoparticle properties. Apart from kinetic constants and affinities, thanks to a multiwavelength scanning setup, also conformation changes can be resolved using LayerSolver. PureKinetics™ (pat.pend.) allows measurements of bulk effect directly in the measurement channel providing clean sensograms without bulk artifacts even in 100% serum or 5% DMSO. The MP-SPR Navi™ instruments can be used for traditional SPR applications and moreover also in material sciences.
The applications include:
(Bio)pharmaceuticals – drug-target, drug-lipid and also drug-living cell interactions, antibody characterization, controlled drug release, nanoparticle drug delivery, small molecules, measurements in 100% serum.
Biosensors – developing assays directly on final material – cellulose for printed biosensors, metal for amperometric and potentiometric sensors, polymers for well-plates, metal nanoparticles for SERS, graphene, CNTs… with crude samples including sea water, saliva, plasma and others.
Biomaterials – MP-SPR measures molecular interactions on even 15 microns thick polymers.
Thin solid films – Barrier coating development for solar cells, antibacterial coatings, moisture barrier, gas sensors using graphene, ceramics, metals and more… measurements of thickness and refractive index down to 3.7 Ångströms (graphene monolayer).
MP-SPR Navi instruments
Currently, there are four models in the MP-SPR Navi family:
The instruments come with PureKinetic feature that measures bulk effect in-line and allows for even 5% changes in DMSO concentration during run or measurements in 100% serum, providing premium quality kinetics data.
LayerSolver is a software module allowing quantification of true thickness and refractive index based on a unique scanning multiwavelength measurement.
The instruments use an elastomer-coated prism which ensures easy handling of sensors (no need for refractive index oil or gold-coated prisms) and also enables storage of the sensors for future experiments, re-use or validation ex-situ.
There is a wide range of optional flow-cells including combinations with electrochemistry, fluorescence or measurements in harsh liquids. The flow-cells can be easily exchanged with a single button push.
SensiQ Technologies offers a full range of Surface Plasmon Resonance instruments. "SensiQ® Discovery" is an entry-level 2 channel manual instrument intended for low-throughput applications. The "SensiQ" is a 2-channel semi-automated instrument with full temperature control, suitable for both affinity and kinetics measurements. "SensiQ Pioneer" is a fully automated and highly sensitive 3 channel capable of analyzing hundreds of samples per day using 96 and 384 well plates.
The latest addition is the SensiQ® Pioneer FE (Fragment Edition) is the new flagship SPR system and includes key features for fragment screening applications. The Pioneer FE is particularly suited to meet this challenge by providing rapid affinity data (KD) from primary screens alone and by enabling prompt identification of fragment candidate actives. Using a combination of dynamic injection SPR (diSPR®) and actives selection software, Pioneer FE provides a significant leap in real-time, label-free identification and characterization of fragment actives in screening campaigns.
Each instrument package includes full Control and Data analysis software packages. SensiQ offers a full line of affordable surface chemistries, allowing a diverse range of biomolecules to be analyzed, including small molecules below 95 Da.
The OneStep (Taylor Dispersion Injections) adds a capillary tube just in front of the sensor and disperses the sample, making it possible to do a gradient injection over 3-4 orders of magnitude. Using the Taylor Equations (from the 1950's) it is then possible to calculate diffusion coefficients and assess aggregation. More importantly, with a derivative of the sigmoidal gradient output, strong and weak binding events are now revealed in time. This has never been accomplished using SPR in the past because the sum of two exponentials is very difficult to resolve (1), (2).
|(1)||Quinn, John G. Evaluation of Taylor dispersion injections: Determining kinetic/affinity interaction constants and diffusion coefficients in label-free biosensing. Analytical.Biochemistry 421: 401-410; (2012). Goto reference|
|(2)||Quinn, John G. Modeling Taylor dispersion injections: Determination of kinetic/affinity interaction constants and diffusion coefficients in label-free biosensing. Analytical.Biochemistry 421: 391-400; (2012). Goto reference|
The ProteOn™ XPR36 Protein Interaction Array System is based on SPR technology and capable of simultanously measurement of 36 individual molecular interactions. It intergrates a high-efficiency crisscross microfluidics system with a high-sensitivity optical system te generate data over the 6 x 6 interaction array, for the analysis of up to six ligands with panels of six analytes.
The system features an optical system that utilizes synchronized sequential scanning illumination coupled with an imaging device to detect the SPR response for each point on the sensor chip.
The ProteOn system measures a total of 78 spots, 36 which represent the interaction data from the 6 x 6 array. The additional 42 spots are called interspots and are used for normalization. The interspots are not exposed to activation or ligand immobilization but are exposed to analyte flow. Both interspot referencing and reference channel subtraction are available with the proteOn system.Visit Bio-Rad
The P4SPR is a unique portable, 4-channel SPR instrument that delivers high-performance measuring and monitoring data of specific targets in complex media such as drugs or disease biomarkers (e.g. antibodies, antigen, hormones) in blood serum or toxic chemicals (e.g. microorganism toxins, industrial organic compounds) in underground waters. The P4SPR's accessible price makes it an attractive label-free system for academic research and startup labs performing assay optimization, system characterization or routine monitoring of proteins and chemicals in lysate, blood, milk or simple buffer. Also, the P4SPR's open architecture makes it easy to combine with other analytical methods such as spectroscopy, chromatography and mass spectrometry to fit your specific needs.
The NanoSPR8 481 is a eight channel Electrochemical Surface Plasmon Resonance Spectrometer capable of performing real time biokinetic, immunosensing and biosensing measurements.
The IBIS MX96 applies SPR imaging with scanning angle optics. The large dynamic and linear range allows label free measurements to be ranging from less than 1 Resonance Unit (RU) up to 30.000 RU with noise levels of 0.2 RU RMSD. The imaging platform facilitates real-time sensing of analyte interactions in combination with the Continuous Flow Microspotter (CFM) of Wasatch Microfluidics (Salt lake city, Utah US) on a 6x8, 4x12 or an 8x12 CFM array of ligands.
Strong feature of the IBIS MX96 is the possibility to apply unlimited exposure times of samples for very low limits of detection. Its valve-less injection of samples and "back-and-forth" flow-based fluidics enables to inject only 70 microliter sample volumes which can be recaptured. The unsurpassed multiplexing capacity of this platform provides scope to use various surface chemistries including 3D (gel-type) and 2D (planar) chemistries on SensEye® sensors.
An analysis cycle of 96 samples of 70 microliters in combination with analysis of a 48 spot microarray, generates > 5000 referenced sensorgrams of unattended operation in a single run. The SPRintX software has been developed to measure biomolecular interactions in such an organized way that kinetic evaluation of the analysis is straightforward. It enables the user to measure high content screening assays without coming into an uncontrolled mess of data which cannot be processed conveniently. The instrument competes with the other instruments in the high-end label free sensing market. It has been sold to various pharmaceutical companies, biotech and research institutes.Visit IBIS
Reichert Technologies - Life Sciences introduces the new Reichert4SPR, 4 Channel Surface Plasmon Resonance system for label-free, real time investigation of biomolecular interactions. Sensitivity and baseline stability make the Reichert4SPR the ideal instrument for both macromolecular and small molecule kinetic or concentration determination experiments. The Reichert4SPR has four channels and is targeted on the typical industrial Biacore T200 user. The four channels can be run with three experimental channels and one reference or with two experimental channels with separate reference.
With a low noise (+/- 0.05 µRIU RMS) and low drift (0.1 µRIU min-1) the instrument is perfect for small molecules or low concentrations of large molecules.
Drag and drop injection, dissociation and regeneration methods make setting up run tables fast and easy. Loop functions simplify repetitive operations on up to 768 samples.
|Technical Specifications: Reichert4SPR|
|Sample loading||Autosampler - up to 768 samples|
|Temperature range||10°C below ambient to 70°C|
|Degassing unit||Built in|
|Flow rate range||0.1-250 µL/min|
|21 CFR Part 11 compliance||Compliant|
|Sample volume||5-5000 µL (depends on loop volume)|
|Refractive index Resolution||< 10⁻⁸ RU|
|Refractive index range||1.33 – 1.40 (@780 nm)|
|Analyte concentration range||1 mM – 1 pM|
|Minimum molecular weight detection||< 100 Daltons|
|Baseline noise||0.05 µRU RMS|
Reichert SR7500DC Dual Channel Surface Plasmon Resonance System.
Reichert's SR7500DC pushes the limits of detection and sensitivity in label-free interaction analysis.
The SR7500DC Dual Channel System is the next generation of surface plasmon resonance (SPR) technology with incomparable sensitivity and high-quality binding data for interaction analysis - all in a flexible component-based platform. The key to the system is the advanced SR7500 Dual Channel SPR Spectrometer instrument.
The SR7500DC label-free system generates data on critical interactions between proteins, nucleic acids, lipids, carbohydrates, small molecules, whole cells, bacteria, viruses, and polymers.
The SR7500DC System is used in pharmaceuticals, drug discovery, antibody screening, protein structure and function, gene regulation, and systems biology. Exceptional sensitivity in the SR7500DC System provides real-time data and is ideal for:
BI-SPR instruments utilize a unique detection technology that delivers high sensitivity and fast response times with superior flexibility. The innovative use of interchangeable analysis modules is specially developed to fulfill a broad spectrum of applications ranging from life science and electrochemistry research to chemical vapor sensing. The BI-4500, a 5-channel series SPR, is equipped with BI-DirectFlowTM technology, which enables precision sample delivery with near-zero dispersion for higher-quality results, ideal for the study of ultra-fast kinetic interactions such as those in DNA sequencing, protein-protein interactions, ligand-receptor recognition, and drug development applications. The BI-4500 system can be combined with the Gas SPR Analysis Module, which helps facilitate chemical vapor detection, gas chemical sensor research, and fundamental solid-gas interface studies. Additionally, the Electrochemical and EC-DualFlowTM Analysis Modules can be easily implemented for electrochemical SPR applications, allowing for anodic stripping analyses and studies on electropolymerization, electrodeposition, redox-related conformational change, and much more. BI-SPR technology can also expand research capabilities by aiding in the development of new high-performance SPR and coupled SPR techniques, made possible by the instrument's open detection stage and auxiliary data ports that facilitate its integration with ongoing research projects and other instruments. BI-SPR instruments produce superior, high-quality data at a completely affordable price.
Key features include: Automated sample delivery with BI-DirectFlowTM Technology; Innovative multi-module design for maximum flexibility in research; Compatibility with electrochemistry applications; Five-channel SPR detection module for background and reference subtraction; Single and serial fluidic modes for more experimental options and enhanced data quality; Wide dynamic range for both large and small molecules (≤ 100 Daltons) and high sensitivity (≤ 10-5 degrees); Broad response time for slow (hours) and fast (≤ 4 ms) kinetic processes.
SENSIA is a company of the Mondragon Group.
SENSIA has been following an innovative process of machine development and is presently bringing into market a new fully automated, bi-channel SPR device, delivering with an unequalled sensitivity and extreme stability. This new machine is a valuable addition to the known brands because of its transportability and robustness.
The main features of the new INDICATOR machine are:
The SPR imaging platform from HORIBA Scientific-GenOptics is adding a new dimension to conventional SPR. Surface Plasmon Resonance imaging (SPRi) combines the advantages of label-free analysis to the throughput of array-based assays. Hundreds of molecular interactions can be monitored in parallel in real-time (multiplexing). Ligands are immobilized in an array format (spots matrix) and the analyte solution is passed over the whole surface of the biochip. The SPR imaging signal is retrieved as real-time kinetic curves (sensorgrams) but also as real-time images of the biochip surface displaying binding events.
The SPRi platform encompasses a complete range of solutions for biomolecular interaction experiments. It includes proprietary sensor chips (SPRi-BiochipTM and SPRi-SlidesTM), an extensive range of surface chemistries (self-assembled monolayers or Dextran-based), printing devices and SPR imaging detection systems.
SPR imaging detection systems
The SPRi-Lab+TM is a flexible and manual instrument suitable for simple operation and easy determination of real-time physico-chemical interactions. Its open format allows combining SPR measurements to other techniques such as electrochemistry or fluorescence.
The SPRi-PlexIITM (photo) is a temperature-controlled semi-automated device equipped with a computer-controlled internal fluidic system. It is the ideal solution for bio-assays such as antibody characterization, immunoprofiling, biomarker discovery. The system can be coupled to the SPRi-autosampler for more demanding applications requiring routine sample injections.
Direct coupling of SPR imaging to MS HORIBA Scientific is pioneering the direct coupling of SPR imaging to MS (mass spectrometry). Thanks to the open format of the SPRi-SlideTM, direct MALDI-MS spectra can be acquired from the biochip, without the need for eluting the analyte.Visit Horiba
The BiOptix 404pi is an affordable phase-based SPR instrument capable of performing high-sensitivity molecular interaction analysis in a high-throughput manner. The 404pi combines the sensitivity and performance of our proprietary technology with an advanced, multi-injector fluidics system.
Features of the BiOptix 404pi include:
Four Injector Design
A unique feature of the BiOptix 404pi is the use of a four needle auto sampler and four parallel flow cells. This allows the operation in a mode that provides four times the throughput of an ordinary SPR instrument, while maintaining the sensitivity that users demand. The 404pi can also be operated in a traditional mode for more detailed analysis of molecular interactions. The 404pi is capable of using vials or 96/384 well plates.
The PlexArrayTM HT System from Plexera is a high throughput, label-free, SPR-based biomolecular interaction detection system for producing high quality information on kinetics, affinities, and specificities of reactants. Advanced optics, specialized software, and PlexArray TM Sensor Chips enable simultaneous measurements of 10's, 100's, or 1000's of unique ligand/analyte interactions on a single, high density array. The PlexArrayTM Data Analysis Software quickly and easily performs parallel analysis of multiplex detections including low affinity and small molecule interactions.
Key Features of the PlexArrayTM HT System
BIACORE 3000 system is equipped with a continuous flow system in which four channels are coupled in series. It has an automatic sample needle to deliver buffer and sample to the sensor chip surface. The continuous flow ensures that no changes in analyte concentration occur during the measurement. By using one of the flow channels as an in-line reference, a perfect control is obtained on the same sample aliquot.
The instruments of Biacore AB use a wedge shaped light source and a diode array to measure the relative outcoming light intensities. In this way, the whole optical interface has no moving parts.
Direct binding of non-labeled components can be monitored in samples ranging from small organic compounds and peptides to eukaryotic cells. Very low analyte concentrations down to the picomolar range can be handled with confidence. Non-specific binding and protein denaturation are both minimized by the use of biocompatible materials with low surface adsorption properties.
The latest machines from Biacore are the T100 and the T200, the successor of the Biacore 3000 and the Biacore A100 aiming at medium throughput. The flow cell design is different because the sensor spot are now dynamically addressed by the flow rate. Each of the four flow cells has five spots so 20 interactions at the same time can be monitored. The Biacore X100 is a simple version of the T100 with two flow cells and wizard guided interaction methods. This machine is simple to learn and simple to use but with the power and precision of a T100.
||The DRX 2400 system of Dynamic Biosensors uses the switchSENSE technology to measure molecular interactions. DNA nanolevers are electrically actuated at high-frequency on microelectrodes, while their orientation is monitored by time-resolved single photon counting. The binding of analyte molecules slows the switching dynamics in a characteristic way, providing an unprecedented level of information about the target.|
|SAW Instruments Gmbh||The sam5 from SAW Instruments uses Surface Acoustic Wave technology (SAW) for label-free detection. By using a bimodal measuring technique, this instrument is able to measure conformational changes in membranes and vesicles. The sensor chips integrate five independent sensor elements.|
|Epic system||The SPR machine handles 384 wells plates. It is capable of measuring binding to proteins, receptors and whole cells.|
|KE-Instruments||Nice range of different SPR instruments (Esprit, Twingle, Springle).|
|Nano SPR||NanoSPR: an eight-channel SPR-instrument and some accessories for SPR.|
|Graffinity||Sells the RAISE platform for fragment library screening. Fragments are between 100 - 300 Da.|
|Xantec bioanalytics - sensors and surfaces||XanTec bioanalytics is dedicated to the development of affordable, customer-orientated innovations in the field of bioanalysis.|
|GWC Technologies||The SPRimager II is an array based SPR machine which produces an real time image of the sensor chip surface instead of a graph.|
Bio-Suplar: a small and light-weight module, idela for training purposes.
|Biosensors - links||Huge amount of links to biosensor companies.|
|Sensor chip surfaces||Dutch company selling sensor chip surfaces for all kinds of machines.|