This is a short quiz about the experimental set-up of experiments. It will take only two minutes to compete. Succes!
1. Why should you use double referencing?
2. Why should you use the 'Kinetic inject' method?
3. Why should duplo’s be in separate capped vials?
to minimize sample dilution to minimize sample evaporation to minimize carry over between samples to minimize sample loss to the vial wall
4. With a slow dissociation, the best set up is to use
multi cycle kinetics single cycle titration kinetics short & long kinetics steady state kinetics
5. Randomization of the samples is done because
it minimizes systematic errors it can reveal systematic errors it proves the system is reproducible it minimizes carry over between samples
6. To get a better estimation of the kinetics you
repeat the method on the same sensor chip use a wide analyte concentration range add more analyte injections in the method repeat the method with different ligand and analyte batches
7. How do you detect mass transport limitation?
by immobilizing less ligand by varying the flow rate during analyte injection by injection several analyte concentrations by varying the analyte injection time
8. What can you try to minimize non-specific binding?
use a low pH running buffer use a higher flow rate lower the analyte concentration adding more salt or detergent to the running buffer
9. What can you do when there is baseline drift?
use a higher flow rate immobilize less ligand flow for a longer period of time with flow buffer ad more salt or detergent to the running buffer
10. What is the best analyte concentration range to inject?
0 – 1 µM 0 – 100% of Rmax 0.1 – 10 times the expected KD at least one concentration should saturate the ligand
11. Replicate analyte injections are done
to prove the system is stable and reproducible to have more curves to analyse to reveal dilution errors to minimize systematic errors
How many legs on a typical dog? (e.g: 5)
