Software

Below software programs which can be of use.

SPR-Simulation

SPR-SimulationThe SPR-Simulation program is a standalone application to simulate kinetic interaction curves. The program draws the curves after the input of the kinetic rate constants, the analyte concentration and the association and dissociation times. Other features are the simulation of equilibrium sensorgrams and plotting an overlay over an imported sensorgram figure.

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BiaMethodWriter

BiaMethodWriterLogoThe BiaMethodWriter is a program to write the Biacore automation methods in a more interactive way. It works for the control software with the Biacore 2000/3000. The new T200 control software is newly designed and not compatible with BiaMethodWriter. This program uses pop-up menus and dialog boxes to fill in the different parts. Remembering these odd parameters is not necessary any more.

The program uses coloured text formatting which gives a better overview of the method and makes it easier to find errors. There is a connection between the method code and the vial places. BiaMethodWriter will fill in the vials in the racks only when they are used in the program.

The report function, will calculate the vial volumes and times used in the method. In addition, there is a summary of the cycles and injections. Both the method and report can be printed. Use a colour printer for the best results.
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KD-Assistant

KDAssistant

The KD Assistant is programmed by Baoxian Li and is a small program to assist with the optimization of kinetics runs. The program can calculate the maximal analyte concentration to be used from one manual three-minute analyte injection. The input consists of the initial binding rate (slope), the response of the analyte injection and the analyte concentration.
The program deals also with the capturing level of the ligand and adjusts the analyte concentration accordingly.

 

Genedata Screener

Genedata logoGenedata Screener® for SPR analyzes surface plasmon resonance data from any instrument. Providing full control over every analysis step, the single-platform analysis system enables interactive adjustments with all dependent calculations automatically recalculated accordingly. Pre-processing is automated and any pre-processing step can then be adjusted from anywhere in the analysis process. Screener for SPR also provides extended diagnostics, graphical overviews, flexible annotations, and outlier rejection, with company-wide access to all experimental data from any SPR instrument. Combining SPR technology with Screener analysis workflow, Screener for SPR standardizes and automates raw data loading, pre-processing, analysis, result generation, and reporting, independent of sample throughput and application type. The workflow is tailored to SPR-specific output and contains built-in business logic to rapidly produce high-quality results. The system is proven to improve productivity by eliminating manual error-prone processes, and enabling faster software performance, centralized set-up and maintenance, and higher utilization of installed instruments.
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Scrubber

scrubberlogoScrubber 2 is a commercial program of Biologic Software. The program can read and write Biosensor files. The purpose of the program is to align and clean the sensorgram data in a structured manner. It can for instance zero in x and y; subtracting reference channels and blanks. Version 2 extends the simple analysis with better models and with options to calculate mass transport rate. The whole sequence of aligning can be recorded and reused again like a macro.
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BiaCalculations

BiaCalcBiaCalculations is an Excel file with the most used calculations in Biomolecular interaction analysis. It contains sections for calculations on conversions, immobilization, equilibrium, flow cell and diffusion. In addition, you can calculate dilution series and do some report-point transformations. And there is a simple simulation of the association and dissociation.
The formulas are explained in the BiaCalculation PDF-file.