Methods and protocols
This section contains links to methods and standard protocols to download. The protocols are designed for the Biacore 2000/3000 instruments.
Click the icon to download the PDF-file or the text for a short description.
|Standard operation protocols|
|Method files for simple immobilizations|
|SPR-Pages Maintenance calendar and some explanation.|
|SPR-Pages standard experiment forms|
These protocols are tasks that are listed in the menu "tools" of the BIACORE Control Software and are mainly focused on the machine and the maintenance of it. Here they are supplemented with some extra protocols.
In the protocol the Thermo racks are listed and the vial positions which will be used during the procedure. Below the racks there is a list of steps/commands. Each line gives the step/command, vial position, solution, volume, time of the step/command and remarks were necessary. At the bottom of the form the composition of the solutions is given.
The whole set of standard methods consist of:
|StartUP||Starting the machine|
|Continue||Leaving the machine for 72 hours|
|Desorb||Once a week cleaning|
|Sanatize||Once a month cleaning|
|Close||Leaving the machine for 5 days|
|Shutdown||Leaving the machine for more than 5 days|
|System check||Check system performance|
|Emergency stop||Stop a method at once|
|Power failure||Recover from power failure|
Method files which use the Biacore Control Software to automate several parts of the immobilization procedure. Extra methods are added, especially in the area of surface checking.
|PreConc||pre-concentration method on CM5 Sensor chip|
|Active||activation of CM5 Sensor chip with EDC/NHS for amine coupling|
|Couple||coupling of ligand to an amine activated Sensor chip|
|Deactiv||deactivation after amine coupling|
|Immob||activation, coupling and deactivation (amine coupling)|
|Blank||create a blank channel by activation and deactivation|
|MassT||test for mass transfer limitation|
|Time||test for time dependent interactions|
|Aldehyde||aldehyde ligand immobilization|
|Amine||amine ligand immobilization|
|Bigloop||amine ligand immobilization on four flow cells|
|Gradient||amine ligand immobilization creating gradient surface|
|Kinetic||kinetic measurement of analyte|
|L_Thiol||ligand thiol immobilization|
|S_Thiol||analyte thiol immobilization|
Checklists for sample preparation. With this list you have the basics steps of preparing the analyte sample..
Checklists for experimental conditions and analysis. Check all the steps from setting up the machine, immobilization, surface performance, doing the experiments and analyzing the results.
Calendar to remind you to do the maintenance in a structured manner. This form can be used as a part of a maintenance logbook.
Forms to fill in your experimental conditions. These forms guide you through all the important conditions so that on a later moment the experiments can be replicated.