Introduction

The aim of this book is to introduce biomolecular interaction analysis in relation to surface plasmon resonance. The theory and the practical approach of preparing samples and sensor chips, performing the experiment and analyzing the results are discussed. The main emphasis will be on the design of proper experiments, processing the data and analyzing the curves in order to obatin reliable results. In addition, examples are given to avoid the common errors made with SPR. Although written with the Biacore™ in mind, the general principles and guidelines will be applicable to comparable SPR instruments.
This over 250 pages book contains more additional information and figures compared to the SPR-Pages. In addition, the book can serve as a reference next to your SPR machine since it is in a convenient A5 size with spiral band.
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Book contents
1 | Introduction to Biomolecular Interaction Analysis |
2 | Kinetics |
3 | Association |
4 | Steady state |
5 | Dissociation |
6 | Regeneration |
7 | Mass transfer |
8 | Getting a feeling for the curves |
9 | Sensor chips |
10 | Sample preparation |
11 | Immobilization |
12 | Experimental setup |
13 | Data fitting |
14 | Kinetic models |
15 | Modelling thin layers |
16 | Checklists |
17 | Calculations |
18 | References |
19 | Resources |
20 | Index |



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