The Octet system from Forté Bio™ is using Bio-Layer Interferometry (BLI) to detect binding of biomolecules in real time. Because BLI only detects binding to the senor surface, there is minimal interference from biological sample media. Proteins can be assayed in cell culture media or crude lysates without interference. The machine can read 8 sensors simultanously which can be pre coated or modified by the customer.
Bio-Layer Interferometry (BLI)
The Octet System utilizes proprietary single-use biosensors with an optical coating layer at the tip of each sensor. This optical surface is coated with a biocompatible matrix that can interact with molecules from a surrounding solution. The Octet instrument shines white light down the biosensor and collects the light reflected back. Reflected light originates from the interface with the optical layer (a) and from the surface of the biocompatible layer (b) where it meets the surrounding solution. When light waves propagate back from the two reflecting surfaces they interact; some wavelengths show constructive interference, others destructive interference. This interference is captured by a spectrometer as a pattern of intensity variation by wavelength with a characteristic profile of peaks and troughs.