There are companies that provide SPR services such as drug discovery, epitope mapping and kinetics.
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Profacgen has organized a team of scientists with extensive experience in the study of PPIs to offer you one-stop service on your PPI project. We can help with your experiment design, carry out your experiments, and perform data analysis. We have established in our lab a series of assays to help with your research, including high throughput interaction screening assays such as Yeast two-hybrid screening and phage display technology, interaction strength and kinetics assays such as Surface Plasmon Resonance (SPR). Each approach has its own advantages and weaknesses in terms of sensitivity and specificity.
Type of techniques used: SPR, ITC, BLI
Creative Biostructure has in the past years, accumulated rich experience in SPR technology and many experimental data, such as bio-macromolecular interactions, antibody screening/characterization, as well as consistency evaluation of protein/antibody. Our team of scientists with a shared scientific background in virology and biophysical technologies provides custom assay development services for coronavirus infection research and supports SPR experimental design and data analysis. We aim to help customers gain faster access to high-quality binding kinetics data so that you can better understand disease pathways, accelerate lead generation and candidate validation.
Type of techniques used: SPR, ITC, BLI, FRET, STD, MST
Beactica is a service provider specialized in molecular interaction analysis by SPR for exploratory drug discovery. Since 2006, Beactica has supported small biotechs and big pharmas globally in assay development, screens for novel hit matter, and characterization of drug leads. All our staff are distinguished scientists with extensive experience from many types of drug target classes. Some client projects have later been published in e.g. PNAS and Nature, and are summarized at our website.
Beactica strive for scientific excellence, quick project turnarounds, and a customer centered service experience. Get in contact for a free quote.
|Biaffin||Kinetics, Thermodynamics, Concentration||SPR|
|Profacgen||Kinetics, Epitope mapping, Quantification||SPR, BLI, ITC|
|Creative peptides||Kinetics, epitope mapping, ligand fishing||SPRi|
|Total Scientific||Kinetics, Thermodynamics||SPR|
|Jubilant Biosys||Kinetics, Fragment screening, Competition assay||SPR|
|Episentum||Kinetics, SPR method development||SPR|
The SPR-Simulation program is a standalone application to simulate kinetic interaction curves. The program draws the curves after the input of the kinetic rate constants, the analyte concentration and the association and dissociation times. Other features are the simulation of equilibrium sensorgrams and plotting an overlay over an imported sensorgram figure.
SPR-Simulation is capable of the following simulations:
- Multi cycle kintics
- Single cycle kinetics
- Equilibrium kinetics
- 1:1 kinetics on a decaying surface
- 1:1 kinetics with mass transfer
The program can export the sensorgrams in Bitmap, SVG and PNG-format. In addition, the curves can be exported in CSV-format compatible with Excel. The result tables can also be copied.
The simulation of interaction curves based on the fitting results can give extra information to improve the experimental set-up.
You can download the manual of the SPR-Simulation program to see if this program can help you with your experiments.
SPR-Simulation only runs on the Windows operating system.
The SPR-Simulation program is available for a small fee. With this fee the SPR-pages are maintained and SPR-Simulation program is further developed.
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Wyatt Technology Corp., based in Santa Barbara, CA, is the leading provider of analytical light scattering instrumentation for macromolecular characterization. While Wyatt detectors are most often used in combination with chromatography for determining absolute molar mass and size of macromolecules and particles in solution, the Calypso™ system is a novel application of light scattering, characterizing macromolecular interactions by means of Composition-Gradient Multi-Angle static Light Scattering (CG-MALS). CG-MALS measurements require no labeling or immobilization, for true free-solution, unmodified equilibrium and kinetic measurements to determine stoichiometry, binding affinity and kinetic rates. CG-MALS also characterizes non-specific interactions general solution-dependent attraction or repulsion between molecules that affect stability, aggregation, crystallization and purification.
LSPR instruments utilize a broad band white light source such as an LED to illuminate the nanoparticle sensor substrate. A spectrometer serves as the detector and can be placed in either a transmission or reflection arrangement. The spectrometer provides a reading of the absorbance spectrum of the sensor substrate. The nanoparticle substrates have a characteristic absorbance peak in the visible spectrum due to the LSPR effect. The properties of this absorbance peak (spectral position, absorbance height) are dependent on the local optical properties surrounding the nanoparticles. When a molecule binds to the surface of the nanoparticle, it changes the local optical properties and causes the absorbance peak to change. The properties of the absorbance peak can be monitoring in real-time, providing time resolved molecular analysis on the nanoscale. The size, shape, and material of the nanoparticles can be tuned in order to maximize sensor performance for specific applications.
Download more information from Nicoya Lifesciences.
OpenSPR by Nicoya Lifesciences utilizes localized surface plasmon resonance (LSPR) for label-free analysis. LSPR is similar to SPR, but it uses nanoparticles of gold rather than a continuous gold film to produce an optical resonance. LSPR’s main advantages are simplicity of the optical design, highly tunable optical properties, no need for temperature control, and robustness. Nicoya has developed a proprietary manufacturing process to create nanoparticle sensors that are highly uniform and stable. Sensors can be functionalized using standard immobilization and coupling chemistry.Visit Nicoya Lifesciences