The aim of this book is to introduce biomolecular interaction analysis in relation to surface plasmon resonance. The theory and the practical approach of preparing samples and sensor chips, performing the experiment and analyzing the results are discussed. The main emphasis will be on avoiding pitfalls and designing proper experiments to obtain reliable results. Although written with the Biacore in mind, the general principles and guidelines will be applicable to comparable SPR instruments.

The book contains more additional information and figures compared to the SPR-Pages. In addition, the book can serve as a reference next to your SPR machine.

Book contents

1	Introduction to Biomolecular Interaction Analysis
2	Sample preparation
3	Sensor chips
4	Pre-concentration
5	Immobilization
6	Association
7	Steady state
8	Dissociation
9	Regeneration
10	Mass transfer
11	Experimental setup
12	Data fitting
13	Kinetic models
14	Checklists
15	Calculations
16	Getting a feeling for the curves
17	References
18	Resources
19	Index

This over 180 pages hardcover book is a valuable resource next to your SPR machine.

Introduction

Book contents

Book corrections